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. 2009 Aug;127(4):477–488. doi: 10.1111/j.1365-2567.2008.02977.x

Figure 2.

Figure 2

Glycosylation-dependent colocalization of Jacalin receptors with CD45 on human primary CD19+ B cells and Raji B cells. (a, b) Expression of Jacalin receptors and CD45 on human CD19+ B and Raji B cells. The separated human CD19+ B cells (a) and Raji B cells (b) were analysed by two-colour flow cytometry using fluorescein isothiocyanate (FITC) -labelled Jacalin and phycoerythrin (PE) -labelled anti-human pan-CD45. The proportions of costained primary CD19+ B cells and Raji B cells are indicated in the upper-right portions of the histograms. The results are representative of three independent experiments. (c, d) Costained primary CD19+ B cells and Raji B cells with Jacalin-FITC and pan-CD45-Alexa546. Human primary CD19+ B cells (c) and Raji B cells (d) were costained with FITC-conjugated Jacalin and anti-human pan-CD45 monoclonal antibodies, followed by Alexa546-conjugated second antibody, in the absence (upper panels) and presence (lower panels) of 100 mm Me-α-Gal, respectively, and then monitored by confocal microscopy. The yellow fluorescence shows the combined fluorescence of FITC (green) and Alexa546 (red). The magnified views of colocalization are shown in right panels.