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. 2009 Aug;127(4):477–488. doi: 10.1111/j.1365-2567.2008.02977.x

Figure 6.

Figure 6

CD45-mediated Jacalin-induced Raji B-cell apoptosis. Raji B cells were treated with Jacalin at 50 μg/ml in the absence or presence of the PTPase CD45 inhibitor for 72 hr, followed by assessment of apoptosis. PARP cleavage was analysed by Western blotting for apoptosis detection. The arrows indicate 116 000 PARP (upper bands) and the 89 000 apoptosis-related cleavage fragment (lower bands). As a positive apoptosis control, Raji B cells were exposed to ultraviolet irradiation at 254 nm for 15 min. GAPDH is shown as a control for protein loading (bottom). The results are representative of three independent experiments.