Figure 4.

Influence of 1α,25-dihydroxyvitamin D₃ [1α,25(OH)₂D₃; D] and Mycobacterium tuberculosis (MTB) on interleukin-10 (IL-10) gene expression and secretion by peripheral blood mononuclear cells (PBMC) and monocytes (MN). 1α,25(OH)₂D₃ had no effect on constitutive IL-10 gene expression in PBMC or MN. Mycobacterium tuberculosis induced IL-10 gene expression in MN at 6 hr postinfection. 1α,25(OH)₂D₃ induced IL-10 gene expression in M. tuberculosis-infected PBMC and MN, and induced IL-10 secretion from PBMC in the presence, but not in the absence, of M. tuberculosis. Ten donors; error bars, SE; **P < 0·01; ***P < 0·001; EtOH = 0·1% ethanol vehicle.