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The PNPA derivatization of LNnT: (a) the chemical reaction equation; (b) Dionex profiles of LNnT−PNPA conjugation over a time course; (c) RP-HPLC profiles of supernatant and precipitate after precipitation of the LNnT−PNPA conjugation with acetonitrile; (d) ESI-MS of the HPLC purified LNnT−PNPA showing the expected mass; (e) MALDI TOF of the starting material and the product of several compounds, showing the expected masses and fragmented major peaks.
