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BSA conjugation with LNnT−PNPA: (a) the general equation of protein conjugation with GPNPAs; (b) overlay of MALDI-TOF profiles of BSA conjugation with LNnT−PNPA at 37 °C in various buffers; (c) fluorescent SEC-HPLC profiles of BSA conjugation with LNnT−PNPA at different temperatures and buffers; (d) overlay of MALDI-TOF profiles of BSA conjugation with lactose−PNPA at different ratios.
