Table 2.
Tryptic Digest of RbcL from tada-1
m/z Observed | z | Mr Calc | Sequence | Ion Score | |
---|---|---|---|---|---|
RbcL | 511.2719 | +2 | 1020.524 | DTDILAAFR41 | 59 |
625.3409 | +3 | 1248.6714 | ESTLGFVDLLR350 | 75 | |
AtpA | 626.8563 | +2 | 1251.6935 | VINALANPIDGR119 | 49 |
737.8978 | +2 | 1473.7787 | ASSVAQVVTSLQER216 | 52 | |
AtpB | 978.0159 | +2 | 1954.016 | (R109)IFNVLGEPVDNLGPVDTR127 | 94 |
736.3841 | +2 | 1470.7541 | VGLTALTMAEYFR261 | 89 | |
1031.0219 | +2 | 2060.0248 | GIYPAVDPLDSTSTMLQPR378 | 69 |
After mass spectrometry analysis of the RbcL band of tada-1, 31 peptides derived from RbcL, AtpB, and AtpA were unambiguously identified. Only peptides corresponding to cleavage at potentially mistranslated Arg residues (in bold) are listed in this table. Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Ions scores >29 indicate identity or extensive homology (P < 0.05). Mr Calc, relative mass calculated from the mass-to-charge (m/z) ratio. The full list of ions observed during this experiment is available in the Supplemental Data Set 1.