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. 2009 Jul;21(7):2058–2071. doi: 10.1105/tpc.109.066654

Table 2.

Tryptic Digest of RbcL from tada-1

m/z Observed z Mr Calc Sequence Ion Score
RbcL 511.2719 +2 1020.524 DTDILAAFR41 59
625.3409 +3 1248.6714 ESTLGFVDLLR350 75
AtpA 626.8563 +2 1251.6935 VINALANPIDGR119 49
737.8978 +2 1473.7787 ASSVAQVVTSLQER216 52
AtpB 978.0159 +2 1954.016 (R109)IFNVLGEPVDNLGPVDTR127 94
736.3841 +2 1470.7541 VGLTALTMAEYFR261 89
1031.0219 +2 2060.0248 GIYPAVDPLDSTSTMLQPR378 69

After mass spectrometry analysis of the RbcL band of tada-1, 31 peptides derived from RbcL, AtpB, and AtpA were unambiguously identified. Only peptides corresponding to cleavage at potentially mistranslated Arg residues (in bold) are listed in this table. Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Ions scores >29 indicate identity or extensive homology (P < 0.05). Mr Calc, relative mass calculated from the mass-to-charge (m/z) ratio. The full list of ions observed during this experiment is available in the Supplemental Data Set 1.