Figure 2.

Phylogenetic Evidence for Plant-Fungi HGT with Weak Topology Support Values but Confirmed by Alternative Topology Tests.

(A) The phylogeny of the phospholipase/carboxylesterase family protein demonstrates moderate support for a fungi-to-plant HGT by PhyML bootstrap methods but weak support by all three other topology support assessment methods. Alternative topology tests gave strong support for the placement of the Selaginella sequences within the specific ascomycete cluster, suggesting an ascomycete-to-Selaginella HGT.

(B) The Selaginella sequences do not form a monophyletic cluster in the phylogeny. To test this, we performed a second analysis focusing the taxon sampling on the branches local to the Selaginella group and increasing character sampling. The phylogenetic trees shown are reduced versions of the full tree topologies (see Supplemental Figure 4 online for full details). The figures are labeled using the same conventions described for Figure 1.