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Effect of RECQL4 on FEN1 flap incision activity. FEN1 incision activity was measured using a 1-nucleotide (A and B) or 10-nucleotide (C and D) flap DNA substrate as described in Materials and Methods. The indicated amount of RECQL4 was added. (B and D) FEN1 incision assays were quantified and mean ± standard error was calculated using data from at least two experiments.
