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. 2009 Jan;27(1):29–39. doi: 10.1634/stemcells.2008-0009

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Copyright © 2009 AlphaMed Press

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Vitrification preserves stemness and differentiation marker expression. (A): Immunofluorescent staining of representative sample S0305 with stem cell/precursor markers (Nestin, Msi-1, Oct-4, and Ki-67) (top panel) and multipotentiality markers (TuJ1, GFAP, and O4) (bottom panel). (Bi): Quantification of data in (A), top panel. (Bii): Quantification of data in (A), bottom panel. p > .05 for all samples pairs, indicating that there was no significant difference between vitrified and nonvitrified samples. Data for all other tumor neurosphere lines are available supporting information Figure 1A, 1B, and supporting information Table 2. Abbreviations: GFAP, glial fibrillary acidic protein; Msi-1, Musashi-1; TuJ1, neuron-specific class III beta tubulin.