Figure 5.
Vitrified neurospheres maintain karyotypic integrity and glioblastoma multiforme hallmarks. Single 2 × 105 cells from dissociated neurospheres were karyotyped by metaphase-fluorescent in situ hybridization (mFISH) analyses according to the manufacturer's instructions (MetaSystems XCyte mFISH). Arrows point to polysomy of chromosome 7 and loss of chromosome 10. Asterisks indicate aneusomy of chromosomes 12 and 13. Data for all other neurosphere lines are shown in supporting information Figure 3.