Figure 2.

¹H NMR spectra of extracts of paired normal and lung lesions.

Flash frozen slices from upper lobe (UL, top panel), lower lobe (LL, middle panel) nodules, and non-cancerous tissues (bottom panel) were taken from the resected lung of the patient shown in Figure 1, and extracted with 10% TCA and ¹H NMR spectra of extracts were recorded at 600 MHz. Metabolite assignments were based on our in-house database, 2-D ¹H TOCSY and ¹H-¹³C HSQC NMR spectra (not shown) (Fan et al. 2008). The three spectra were normalized in terms of tissue weight and NMR spectral parameters for direct comparison.