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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Clin Immunol. 2009 Apr 23;132(1):116–123. doi: 10.1016/j.clim.2009.03.517

Figure 1.

Figure 1

A. Sample scatter plots are shown illustrating the identification of whole blood iNKT cell populations. After gating on the lymphocyte population (identified by forward and side light scatter) and CD3+ lymphocytes (T cells), iNKT cells were identified and quantified in all control and patient samples by co-expression of TCRVα24 and TCRVβ11, and in selected samples also by recognition of PBS57-loaded CD1d tetramer. As shown here, simultaneous staining with TCRVα24 and TCRVβ11 and PBS57-loaded CD1d tetramer demonstrates that both methods identify the same population. B. iNKT cell populations were studied in 7 randomly selected control samples using both co-expression of TCRVα24 and TCRVβ11 and by recognition of PBS57-loaded CD1d tetramer to aid in the validation of each method. Results as listed.