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Single-turnover kinetics of the eUNG reaction measured by the rapid chemical quench method and processed using the piperidine-induced abasic site cleavage assay. The data points were fit to a first-order exponential decay (see Table 1 for rate constants). (A) Reaction of 10 μM wild-type UNG with +2 MeP-substituted U dsDNA (20 nM) compared with the phosphodiester form (OxP). (B) Reaction of 10 μM wild-type UNG with +1 MeP-substituted U dsDNA (20 nM) compared with the OxP form.
