. Author manuscript; available in PMC: 2009 Aug 20.

Published in final edited form as: Biochemistry. 2008 Jul 25;47(33):8614–8622. doi: 10.1021/bi800854g

Table 2.

Effects of Methylphosphonate Substitution on Uracil Glycosidic Bond Cleavage by eUNG

DNA	k_cl(ssDNA) (s⁻¹)^a	k_cl(dsDNA) (s⁻¹)	k_cl^OxP/k_cl^MeP (ssDNA)^a	k_cl^OxP/k_cl^MeP(dsDNA)
OxP	110.0	100.3	–	–
+ 1 MeP^late	2.1	0.56	50	180
+ 1 MeP^early	0.26	2 × 10⁻⁷	420	5 × 10⁸
+2 MeP^late	220	54	0.5	1.8
+2 MeP^early	220	180	0.5	0.6

Rates of uracil cleavage in ssDNA were previously reported in ref 2.