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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Kidney Int. 2009 Jan 28;75(7):719–726. doi: 10.1038/ki.2008.697

Figure 4.

Figure 4

Effect of tacrolimus and calcineurin autoinhibitory peptide on eNOS Ser1177 phosphorylation. Tacrolimus (TAC; 1 µmol/L) decreased both basal and acetylcholine-induced eNOS Ser1177 phosphorylation which was reversed by the cPKC inhibitor Gö6976 (1 µmol/L). Calcineurin autoinhibitory peptide (CAIP; 10 µmol/L) had no effect on eNOS Ser1177 phosphorylation. A, Representative Western blots showing basal and acetylcholine-induced aortic eNOS Ser1177 phosphorylation and eNOS expression. B, Densitometry for ratio of eNOS Ser1177 phosphorylation to eNOS expression as a percent of control. Results are expressed as mean ± SEM (n = 3 pooled aortas for each group and 3 independent experiments). *p<0.05 vs control.