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. 2009 Aug 3;8:183. doi: 10.1186/1475-2875-8-183

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Copyright © 2009 Bergmann-Leitner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anti-MSP-1p42 specific sera act to inhibit either merozoite invasion or parasite growth, depending on the parasite clone. Responses of 3D7 (Panel A) and FVO (Panel B) parasites to various anti-MSP-1p42 antisera (at 20% v/v) were tested and evaluated by determining inhibitory activity of the sera based on total parasitaemia (Syto-16; black bars) and viable parasitaemia (HE, white bars). Test sera used: MSP-1p42 (FVO)(FMP003, FMP010) or MSP-1p42 (3D7) (FMP1) in Freund's adjuvant (FA) or FMP003 and FMP010 in Montanide ISA 720. Data are the mean and SEM of three independent experiments. The ELISA data are expressed as OD 1 titers against the MSP-1p42(3D7) (Panel A) or MSP-1p42 (FVO) (Panel B) plate antigen.