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. 2009 Aug 3;8:183. doi: 10.1186/1475-2875-8-183

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Copyright © 2009 Bergmann-Leitner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anti-MSP-1p42 antisera prevent the rupture of heterologous and homologous clones, FVO and CAMP/FUP, but not 3D7. Cultures were set up with schizont stage pRBCs in the presence of sera (at 20% v/v) from rabbits immunized with either MSP-1p42(3D7) (FMP1) or MSP-1p42(FVO) (FMP003) emulsified in FA. Sera from rabbits immunized with reduced/alkylated MSP-1p42(R/A)/FA were used as the negative control. Triplicate blood smears were prepared from each culture 6 hrs after invasion was completed. Micrographs of representative parasites were taken at 1000× magnification.