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. 2009 Aug 3;8:183. doi: 10.1186/1475-2875-8-183

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Copyright © 2009 Bergmann-Leitner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anti-sera raised to MSP-1p42 treated parasite cultures results in retardation of parasite development. 3D7 (panel A) and FVO (panel B) schizonts were cultured in the presence of control serum or immune rabbit serum (at 20% v/v) raised against either reduced/alkylated MSP-1p42 (R/A, control serum) or MSP-1p42(3D7) (FMP1) or MSP-1p42(FVO) (FMP003) emulsified in FA. Parallel cultures were harvested at indicated time points after completed rupture and slides prepared in triplicates per treatment group and time point. Micrographs of representative parasites in Giemsa-stained blood smears were taken at 1000× magnification.