Table 1.
Quantitative analysis of Giemsa-stained blood smears of cultures incubated with various antisera.
| (A) 6 hrs post invasion | ||||
| Test clone | Antisera testeda | Proportion of phenotype [%]b | ||
| rings | schizonts | agg mz | ||
| 3D7 | Control (R/A) | 100 | 0 | n |
| Anti-MSP-1p42(3D7) | 100 | 0 | y | |
| Anti-MSP-1p42(FVO) | 100 | 0 | y | |
| FVO | Control (R/A) | 98.8 | 1.2 | n |
| Anti-MSP-1p42(3D7) | 70.0 | 30.0 | y | |
| Anti-MSP-1p42(FVO) | 65.4 | 34.6 | y | |
| CAMP/FUP | Control (R/A) | 97.1 | 2.9 | n |
| Anti-MSP-1p42(3D7) | 79.2 | 20.8 | y | |
| Anti-MSP-1p42(FVO) | 74.3 | 25.7 | y | |
| (B) 12 hrs post invasion | ||||
| Test clone | Antisera testeda | Proportion of phenotype [%]b | ||
| rings | schizonts | agg mz | ||
| 3D7 | Control (R/A) | 100 | 0 | n |
| Anti-MSP-1p42(3D7) | 100 | 0 | y | |
| Anti-MSP-1p42(FVO) | 100 | 0 | y | |
| FVO | Control (R/A) | 99.4 | 0.6 | n |
| Anti-MSP-1p42(3D7) | 73.4 | 26.6 | y | |
| Anti-MSP-1p42(FVO) | 61.9 | 38.1 | y | |
| CAMP/FUP | Control (R/A) | 96.6 | 3.4 | n |
| Anti-MSP-1p42(3D7) | 75.2 | 24.8 | y | |
| Anti-MSP-1p42(FVO) | 70.1 | 29.9 | y | |
a Antisera were tested at 20% (v/v) serum concentration. The control group R/A consists of pooled sera from rabbits immunized with reduced/alkylated MSP-1p42 in FA (see Materials and Methods).
b Analysis was done by counting sufficient fields to accumulate either 2,000 RBCs or 100 pRBC. The pRBC were classified and counted based on their phenotype. No attempts were made to count agglutinated merozoites (agg mz) and thus only their presence in the culture was noted as y, yes and n, no. Data shown in the table correspond to the data shown in Figure 2.