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Contribution of PDE5 activity to total cGMP-hydrolytic activity in subcellular fractions of mouse myocardium. cGMP-hydrolytic activity was measured at 0.1 μM cGMP in the presence of 100 μM EGTA (Ca2+/CaM-independent activity) and in the absence or presence of 10 or 1000 nM (1 μM) sildenafil. Each value represents the average ± S.D. of at least three determinations. N.S., not significant. *, P < 0.05.
