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. 2009 Jun 22;330(3):884–891. doi: 10.1124/jpet.109.154468

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Fig. 7. — Effect of sildenafil on Ca²⁺/calmodulin-stimulated cGMP-hydrolytic activity in cytosolic preparations from mouse myocardium. cGMP-hydrolytic activity was measured at 0.1 μM cGMP in the presence of 200 μMCa²⁺ and 50 nM calmodulin or in the presence of 100 μM EGTA, and in the absence or presence of 10 or 1000 nM (1 μM) sildenafil. The difference was taken as Ca²⁺/calmodulin-stimulated activity. Each value represents the mean ± S.D. of at least three determinations. N.S., not significant. *, P < 0.05.