Fig. 9.

Immunodetection of PDE5 protein in preparations of mouse and human left ventricular myocardium. Cytosolic fractions of mouse tissue (one preparation each from 25 to 50 normal and 25 to 50 failing hearts) and human tissue (two preparations each from three to four normal and three to four failing hearts) were analyzed by Western blotting with anti-PDE5 antibody as described under Materials and Methods. Fifty micrograms of protein were loaded into each lane before SDS-polyacrylamide gel electrophoresis, electrophoretic transfer, and overlay with anti-PDE5.