Figure 5.

Illustration of small RNA cloning incidence in FFPE specimens. A: RNA cloning incidence of the sequenced fragments showed a high fraction (∼80%) of rRNA (red bar in +SLN, before) before adding a complimentary oligonucleotide sequence. Using this sequence, the synthesis of the rRNA fraction (∼20%) was reduced (red bar in +SLN, after) fourfold. This strategy uncovered a higher fraction of small RNAs for analysis. B: A summary of all RNA species is depicted in all four specimens: skin, PCM, −SLN, and +SLN.