Figure 2.

A: Signal-to-noise ratio of molecular beacons. The signal-to-noise ratio of M. tuberculosis and M. bovis beacons was determined by the visual format assay. Tube 1, buffer (negative control); tube 2, buffer + M. tuberculosis beacon (negative control); tube 3, buffer + M. tuberculosis beacon + target oligonucleotide of M. tuberculosis (positive control); tube 4, buffer + M. tuberculosis beacon + target oligonucleotide of M. bovis; tube 5, buffer (negative control); tube 6, buffer + M. bovis beacon (negative control); tube 7, buffer + M. bovis beacons + target oligonucleotide M. bovis (positive control); tube 8, buffer + M. bovis beacon + target oligonucleotide M. tuberculosis. B: Thermo-denaturation profile for molecular beacons. Dotted line denotes M. bovis beacon with its target oligonucleotide melt curve. Continuous line denotes M. tuberculosis beacon with its target oligonucleotide melt curve. All other lines denotes various controls.