Table 2.

Repeat HPV-QUAD Results for Specimens Reported as Negative (<2.0) but with Intermediate Signal Ratios (>1.0 <2.0)

Specimens with positive repeat results
			Run 2		Run 3
Specimen #	HPV type(s)	Run 1* Ratio	Ratio	Result	Ratio	Result
1	52/58	1.3	2.6	pos
2	31	1.0	10.4	pos
3	35/68	1.4	3.3	pos
4	35/68	1.6	2.2	pos
5	6/11	1.9	2.5	pos
6	35/68	1.6	2.2	pos
7	52/58	1.7	4.5	pos
8	52/58	1.6	2.6	pos
9	31	1.5	4.4	pos
10	39/56	1.9	1.9	neg
	51/59	1.2	2.1	pos
	52/58	1.7	2.5	pos
11†	18	0.0	8.6	pos	0.0	neg
	51/59	1.5	7.2	pos	3.1	pos
12	16	0.4	3.4	pos
	39/56	1.1	11.6	pos
13	16	1.0	4.1	pos
	18	1.8	0.0	neg
	39/56	1.2	3.4	pos

Specimens with negative repeat results
			Run 2
Specimen #	HPV type(s)	Run 1 Ratio	Ratio	Result
14	35/68	1.1	1.0	neg
15	51/59	1.3	1.7	neg
16	39/56	1.4	0.4	neg
17	6/11	1.4	1.7	neg
18‡	18	1.6	1.9	neg/IND

All specimens were positive in the HC2 assay, except Specimen 13.

^*All Specimens in Run 1 were reported as negative with HPV ratios <2.0.

^†The result for Specimen 11on Run 2 indicated the presence of HPV18. This was likely contamination and was not present in Run 3.

^‡Specimen 18 was indeterminate (IND) on Run 2 (insufficient beta globin amplification).