Table 3.

Resolution of Hybrid Capture 2 (HC2) Positive/HPV-QUAD Negative Specimens

	Likely false positive HC2	Cross-reactive HC2	Cross-reactive HC2	False negative HPV-QUAD	False negative HPV-QUAD low HPV ratios*
HPV-QUAD result: (# specimens)	Negative (5)	Negative (10)	HPV6/11† (2)	Negative (3)	HPV39/56 (1) HPV35/68 (2) HPV51/59 (1)
Real-time PCR‡ Cp:	>40	26.1 to 38.1	25.6 to 21.2	37.1 to 40.5	29.0 to 39.6
Tm:	None	79.8 to 81.8°C	81.3 to 81.2°C	78.5 to 79.4°C	78.1 to 79.8°C
Sequencing result:	No HPV	HPV53 (2)	HPV53	HPV16	HPV56
		HPV66 (5)	HPV11	HPV56 (2)	HPV35 (2)
		HPV70			HPV59
		HPV64
		HPV82

^*HPV ratios (R) were 39/56 r = 0.73, 35/68 r = 1.22, 35/68 r = 1.11, 51/59 r = 1.31.

^†HPV6/11 are low risk HPV types.

^‡Real-time PCR and sequencing performed using MY consensus primers as described in the Materials and Methods. Cp, crossing point; Tm, melting temperature.