Fig. 2.

Bovine blastocyst morphology before and after vitrification and viability assay of embryonic cells in fully recovered blastocysts. a Day 8 bovine blastocysts produced in vitro. b Collapsed blastocyst during vitrification procedure. c Vitrified/warmed blastocysts after 12 h (c) of culture. d A fully recovered hatched blastocyst. e Differential viable staining of the survived hatched blastocysts with viable (blue) and dead (red) cells. e Tunnel assessment of the hatched blastocyst indicating apoptotic (yellow color) within the total cells (red) of the blastocyst