. 2009 Jun 19;26(6):355–364. doi: 10.1007/s10815-009-9317-7

Table 1.

Effect of βME supplementation on developmental ability of bovine in vitro produced (IVP) embryos

IVP culture design	Presumptive zygotes (n)	Proportion of embryos developed to¹						Quality analysis of blastocysts
IVP culture design	Presumptive zygotes (n)	Cleavage	8–16 cell	Morula	D7 Bls	D8 Bls	D9–10 Hatching	TCN (n)	Viability²(%)	DNA-fragmented³ (%)
IVC ^{+ +}	1,250	92.0 ± 3.1	87.0 ± 2.3^a	75 ± 3.1	28.3 ± 2.2^a	40.1 ± 1.1^a	83.3 ± 3.6^a	248.0 ± 4.5^a	96.3 ± 0.3	6.1 ± 0.0^a
IVC^{+ −}	1,250	92.0 ± 3.1	87.0 ± 2.3^a	75 ± 3.1	28.3 ± 2.2^a	40.1 ± 1.1^a	75.0 ± 4.0^ab	170.6 ± 5.5^ab	94.0 ± 1.0	9.2 ± 0.3^ab
IVC^{− +}	1,325	75.1 ± 2.5	66.0 ± 3.5^b	68 ± 1.5	17.1 ± 1.4^b	33.3 ± 2.3^b	61.1 ± 4.5^ab	168.4 ± 3.8^ab	93.5 ± 0.0	10.4 ± 0.2^ab
IVC^{− −}	1,325	75.1 ± 2.5	66.0 ± 3.5^b	68 ± 1.5	17.1 ± 1.4^b	33.3 ± 2.3^b	38.9 ± 3.3^b	163.7 ± 5.8^b	90.8 ± 1.0	15.1 ± 0.9^b

1: Ratios are expressed based on the number of presumptive zygotes.

2: Percentage of live cells/TCN

3: Percentage of TUNEL-positive/TCN

Different letters within a column are significantly different between treatments (P < 0.05)