Table 2.
Effect of BME supplementation of culture medium (IVC+vs. IVC¯) and/or post-warming medium (PW+vs. PW¯) on cryosurvival of bovine IVP blastocysts
| Comparisons | Survived embryos (%) | Post-warming re-expansion after 12 h (%) | Total hatching (%) | Total degeneration (%) | Quality assay of blastocysts | ||
|---|---|---|---|---|---|---|---|
| TCN (n) | Viability 1 (%) | DNA fragmented 2 (%) | |||||
| IVC+/PW+ | 15.7 ± 1.5a | 84.3 ± 1.6a | 74.3 ± 2.0a | 25.7 ± 3.0a | 170.4 ± 4.2a | 95.2 ± 1.0a | 10.0 ± 1.0a |
| IVC+/PW − | 16.7 ± 1.0a | 83.3 ± 1.0a | 50.0 ± 2.4a | 50.0 ± 2.5b | 160.4 ± 4.5a | 93.4 ± 0.0a | 18.5 ± 1.5b |
| IVC−/PW+ | 15.0 ± 1.1a | 85.0 ± 1.1a | 57.8 ± 1.2a | 42.2 ± 2.2b | 168.0 ± 5.0a | 94.5 ± 1.0a | 13.5 ± 0.2a |
| IVC−/PW − | 35.9 ± 2.4b | 64.2 ± 1.1a | 48.1 ± 3.2b | 51.9 ± 2.3b | 158.0 ± 3.8a | 88.2 ± 2.0a | 20.6 ± 0.5b |
1 : Percentage of Live cells/TCN
2 : Percentage of TUNEL-positive/TCN cells nuclei
Different letters within a column are significantly different between treatments (P < 0.05)