Table 3.
Comparison on different cryosurvival parameters of vitrified vs. non vitrified embryos cultured at the same conditions of βME supplementation
| Parameters | Comparisons | |||||||
|---|---|---|---|---|---|---|---|---|
| IVC+/PW+vs. IVC+ + | IVC+/PW −vs. IVC+ − | IVC−/PW+vs. IVC− + | IVC−/PW −vs. IVC− − | |||||
| Values | LD# | Values | LD | Values | LD | Values | LD | |
| Hatching (%) | 74.3 ± 2.0 vs. 83.3 ± 3.6 | 9.0 ± 1.6 a | *50.0 ± 3.4 vs. 75.0 ± 4.0 | 25.0 ± 0.6 b | 57.8 ± 4.2 vs. 61.1 ± 4.5 | 3.3 ± 0.3 a | 31.4 ± 3.3 vs. 38.9 ± 3.3 | 7.5 ± 0.0 a |
| TCN (n) | *170.4 ± 4.2 vs. 248.0 ± 4.5 | 77.6 ± 0.3 a | 160.4 ± 4.5 vs. 170.6 ± 5.5 | 10.0 ± 1.0 a | 168.0 ± 5.0 vs. 168.4 ± 3.8 | 0.4 ± 1.2 a | 158.0 ± 3.8 vs. 163.7 ± 5.8 | 5.7 ± 2.0 a |
| Viability (%) | 95.2 ± 1.0 vs. 96.3 ± 0.3 | 0.1 ± 0.3 a | 93.4 ± 0.0 vs. 94.0 ± 1.0 | 0.6 ± 1.0 a | 94.5 ± 1.0 vs. 93.5 ± 0.0 | 1.0 ± 1.0 a | 88 ± 2.0 vs. 90.8 ± 1.0 | 2.8 ± 1.0 a |
| DNA-fragmentation(%) | 10.0 ± 1.0 vs. 6.1 ± 0.0 | 5.9 ± 1.0 a | *18.5 ± 0.5 vs. 9.2 ± 0.3 | 9.3 ± 0.2 b | 13.5 ± 0.2 vs. 10.4 ± 0.2 | 3.1 ± 0.0 a | 20.6 ± 0.5 vs.15.1 ± 0.9 | 5.5 ± 0.4 a |
# Level of difference between the two values compared
*Indicates significant difference between non-vitrified vs. Vitrified values of a given comparison (P < 0.05)
In each row, comparisons with different letters are significantly different (P < 0.05)