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. 2006 Aug 22;21(4):627–632. doi: 10.3346/jkms.2006.21.4.627

Fig. 2.

Fig. 2

HNA-5a genotyping by Bsp1,286 I allele-specific restriction enzyme analysis (ASRA). Lane 1 shows a DNA ladder marker (Bioneer, Daejeon, Korea); lane 2 shows an undigested 709 bp PCR product of the αL chain of β2integrin cDNA; lane 3 shows an HNA-5a+ homozygote sample (297 bp, 217 bp, and 195 bp); lane 4 shows a HNA-5a heterozygote samples (412 bp, 297 bp, 217 bp, and 195 bp); and lane 5 shows a HNA-5a- homozygote sample (412 bp, and 297 bp).