Fig. 3.
Granulocyte antibody test using the mixed passive hemagglutination assay (MPHA). The neonates' and mothers' sera were tested using extracted granulocyte antigen-coated microplates (from six donors) as a solid phase and sheep RBCs coated with rabbit F(ab')2 anti-human IgG as indicator cells. To differentiate granulocyte antibody from HLA antibody, sera (left half) with positive reactions were compared with HLA antibody adsorbed sera (right half). In the sera of neonate 1 (N1) and her mother (M1), anti-HNA-1a; in the sera of neonate 2 (N2) and his mother (M2), anti-HNA-1b; in the sera of neonate 3 (N3) and his mother (M3), anti-HNA-1b and anti-HLA.
P, pasitive control; N, negative control.