Figure 7. itr-1, egl-8, and plc-3 all function in nose touch–induced Ca²⁺ transients in ASH.

Ratio changes in cameleon-expressing ASH neurons, following nose touch. (A) Quantification of responses. Diamonds are individual observations; longer red lines are mean; error bars are s.e.m. (n = 20). (B) Representative responses, for wild type animals and the mutants indicated. Black bar indicates duration of stimulation. The CFP/YFP ratio decreases over the course of the recordings because YFP photobleaches faster than CFP; noise is relatively low in some animals, due to higher cameleon expression levels. Nose touch–evoked Ca²⁺ transients were significantly disrupted in itr-1, egl-8, and plc-3 loss-of-function animals (P<0.05, Mann-Whitney rank sum test).