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. 2009 Aug 31;4(8):e6869. doi: 10.1371/journal.pone.0006869

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Nishide et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Proliferation of the GICs-LD and their parental cells (NSCs) was determined by BrdU incorporation assay. The results shown are the mean±SD of three cultures. *P<0.05. (B) The proportion of neural lineage marker-positive cells is shown as the mean±SD of two cultures. *P<0.05; **P<0.01. (C) Colony formation ability was examined in soft agar. (D) Brain sections with tumors derived from GICs-LD were stained with hematoxylin and eosin (HE) and for Nestin, GFAP, MBP, and NeuN. Scales, 2 mm (upper left panel), 100 µm (upper right panel), and 200 µm (middle and lower panels).