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. 2009 Aug 31;4(8):e6869. doi: 10.1371/journal.pone.0006869

Figure 6. Prom1-expressing cells are dispensable for maintaining GICs in culture.

Figure 6

(A) X-gal staining of monolayer-cultured GICs-LD (Monolayer), their tumor spheres (Sphere), and valproic acid (VPA, 10 mM)-treated cells (lower panels; representative data from three cultures). (B) VPA induced TUNEL-positive cell death in GICs-DTA. All nuclei were counterstained with DAPI (blue). Scales, 100 µm (C) Survival of GICs-LD and GICs-DTA was determined by MTT assay. The results shown are the mean±s.e.m of three cultures. *P<0.001. (D) Sphere formation of GICs-LD and GICs-DTA. Upper schemes indicate the structures of the genome. Scale, 60 µm.