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. 2009 Sep 4;5(9):e1000572. doi: 10.1371/journal.ppat.1000572

Figure 6. Klapan-Meier embryo survival curves following infection with P. aeruginosa.

Figure 6

(A) Dose-dependent response in embryo survival to P. aeruginosa infection. Groups of 28–30 hpf embryos (n = 20 embryos/group) were inoculated with a low dose (100 CFU), an intermediate dose (650 CFU), or a high dose (1300 CFU) of PAO1 wild-type cells. Uninfected control is shown (non-injected). (B) Embryo survival following infection with ∼500–800 CFU of P. aeruginosa wild-type strain or with the PA0676 vreR sigma factor regulator mutant bearing the empty pMMB67EH plasmid or the pMUM3 plasmid in which the PUMA3 system is induced by overexpression of vreI. The data are representative of 3 replicates with 20 embryos/group in each replicate. (C) Embryo survival after infection with ∼700 CFU of P. aeruginosa wild-type strain or with the mutants PA0676 vreR, PA0690 tpsA, PA0692 tpsB, PA0695 tonB-homologue, and PA0696 gene bearing the pMUM3 plasmid overexpressing the vreI ECF sigma factor were microinjected in zebrafish embryos. The data are representative of 2 replicates with 20 embryos/group in each replicate.