Table 2. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristicsa | References |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR1 recA1 endA1 gyrA96 thi1 relA1 | [61] |
| HB101 | supE44 hsdS20 recA13 ara14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | [62] |
| P. aeruginosa | ||
| PAO1 | Wild-type | [54] |
| PA0676/vreR | PA0676::ISphoA/hah derivative of PAO1 (ID 42533); TcR | [54] |
| PA0690/tpsA | PA0690::ISlacZ/hah derivative of PAO1 (ID 14608); TcR | [54] |
| PA0692/tpsB | PA0692::ISlacZ/hah derivative of PAO1 (ID 10896); TcR | [54] |
| PA0695/tonB | PA0695::ISlacZ/hah derivative of PAO1 (ID 10838); TcR | [54] |
| PA0696 | PA0696::ISlacZ/hah derivative of PAO1 (ID 8944); TcR | [54] |
| Plasmids | ||
| pBBR1MCS-5 | GmR; oriTRK2 | [60] |
| pBSL99 | Source of the Km cassette; ApR, KmR | [63] |
| pMMB67EH | IncQ broad-host range plasmid, lacIq; ApR | [64] |
| pMP220 | IncP broad-host-range lacZ fusion vector; TcR | [56] |
| pRK600 | Helper plasmid, oriColE1, mobRK2, traRK2; CmR | [57] |
| pRP270 | GST (gluthathione-S-transferase) fusion vector, ApR | [65] |
| pUC18 | Cloning vector, oriColE1, rop mutant, α-lacZ | [66] |
| pUCMA3 | pUC18 carrying in SmaI a 2.2-kb PCR fragment containing the P. aeruginosa PAO1 PA0675-PA0676 genes; ApR | This study |
| pBBR-PoprF | pBBR1MCS-5 carrying in XhoI-HindII a 300-bp PCR fragment containing the P. aeruginosa PAO1 oprF promoter region | This study |
| pMUM3 | pMMB67EH carrying the 1.4-kb KpnI-NheI insert from pUCMA3 containing the PA0675 gene; ApR | This study |
| pMUM3Rσ | pMMB67EH carrying in EcoRI-BamHI a 1.46-kb PCR fragment containing the P. aeruginosa PAO1 PA0674-PA0675 genes; ApR | This study |
| pMMB-PUMA3 | pMMB67EH carrying in EcoRI-BamHI a 2.6-kb PCR fragment containing the P. aeruginosa PAO1 PA0674-PA0675-PA0676 genes; ApR | This study |
| pMUM3Rσno-stop | pMMB67EH carrying in EcoRI-XbaI a 1.45-kb PCR fragment containing the P. aeruginosa PAO1 PA0674-PA0675 genes, the last one without stop codon; ApR | This study |
| pMUM3RσHA-tag | pMUM3Rσno-stop carrying the HA-tag epitope in the C-terminal end of PA0675; ApR | This study |
| pUCPA0692 | pUC18 carrying in SmaI-HindIII a 2.0-kb PCR fragment containing the P. aeruginosa PAO1 PA0692 gene, ApR | This study |
| pUCPA0697 | pUC18 carrying in EcoRI-HindIII a 848-bp PCR fragment containing the P. aeruginosa PAO1 PA0697 gene, ApR | This study |
| pGST-0690 | pRP270 carrying in BamHI-EcoRI a 653-bp PCR fragment containing the P. aeruginosa PAO1 C-terminal region of the PA0690 gene fused to GST, ApR | This study |
| pGST-0692 | pRP270 carrying the 567-bp NcoI-EcoRI insert from pUCPA0692 containing an internal fragment of the PA0692 gene fused to GST, ApR | This study |
| pGST-0697 | pRP270 carrying the 426-bp PstI-ApoI insert from pUCPA0697 containing an internal fragment of the PA0697 gene fused to GST, ApR | This study |
| pMP0691b | PA0691 promoter fragment cloned upstream of lacZ gene in pMP220; TcR | This study |
| pMP0691bKm | pMP0691b carrying in BglII, and in the opposite direction to the lacZ gene, a 1.2-kb BamHI fragment of pBSL99 containing a Km cassette; KmR, TcR | This study |
| pMMB674HA | pMMB67EH carrying in EcoRI/HindIII a 0.8-kb PCR fragment of vreA (PA0674) with a C-terminal HA tag; AmpR | This study |
a
ApR, CmR, KmR, and TcR, resistance to ampicillin, chloramphenicol, kanamycin, and tetracycline, respectively.