Fig. 5.

Truncated PaxG, without the C-terminus tripeptide, does not complement the ΔpaxG mutant strain SSG2 for paxilline biosynthesis. a C-terminal truncated version of the paxG ORF. Numbers indicate amino acid residues. b Expression analysis of SSG2 derivative strains containing PaxG or N368PaxG under the control of the P. paxilli paxM promoter. RT-PCR analysis of total RNA isolated from 6 day old mycelia of P. paxilli SSG2 derivative strains PN2664 and PN2665 containing the constructs pSS110 (PaxG) and pSS111 (N368PaxG), respectively. These strains were confirmed by PCR to contain the respective genes. Numbers on the right correspond to the fragment sizes indicated in kb. c Reverse-phase HPLC analysis of mycelia extracts of P. paxilli ΔpaxG mutant strain SSG2 (PN2662), SSG2 derivative strains PN2664 (SSG2/PaxG) and PN2665 (SSG2/N368PaxG), and wild-type (wt)