Fig. 8.

Full-length and truncated versions of ggsA ORF with the added PTS1 motif SKL do not provide the paxG function for paxilline biosynthesis. a Expression analysis of SSG2 derivative strains containing GgsA-SKL and C304GgsA-SKL under the control of the P. paxilli paxM promoter. RT-PCR analysis of total RNA isolated from 6 day old mycelia of P. paxilli SSG2 derivative strains PN2666 and PN2667 containing the constructs pSS112 (GgsA-SKL) and pSS113 (C304GgsA-SKL), respectively. These strains were confirmed by PCR to contain the respective genes. Numbers on the right correspond to the fragment sizes indicated in kb. b Reverse-phase HPLC analysis of mycelia extracts of P. paxilli ΔpaxG mutant strain SSG2 (PN2662), SSG2 derivative strains PN2666 (SSG2/GgsA-SKL) and PN2667 (SSG2/C304GgsA-SKL), and wild-type (wt)