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. 2009 Sep 8;7(9):e1000189. doi: 10.1371/journal.pbio.1000189

Figure 1. Responsiveness to CLN3.

Figure 1

Various mutants containing bck2 and CLN3 under the control of the GAL promoter (GAL-CLN3) as the only allele of CLN3 were grown in YEP raffinose medium (CLN3 off, solid grey line), split into two aliquots, and galactose was added to one aliquot (CLN3 on, dotted black line). Cell volume distributions were measured with a Coulter Channelyzer (left column), and photomicrographs were taken (right two columns). The median cell size (fl) as measured by the Coulter Channelyzer is shown in the bottom left corner of each photograph, and the percentage budding is shown in the bottom right corner. A shift to smaller cell sizes and to higher percent budding in galactose shows responsiveness to CLN3. The changes in budding are statistically significant at the p<0.01 level for all strains except the whi5 stb1 strain (4th from top) and the whi5 sin3 strain (5th from top). The cell size distribution is not shown for GAL-CLN3 bck2 cells in raffinose (top panel) because these extremely large cells are off-scale. Strains used, from top to bottom, are: LC517, LC520, LC518, LC504, LC524, LC521, and LC523.