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. 2009 Sep 8;7(9):e1000189. doi: 10.1371/journal.pbio.1000189

Figure 6. Cln3 associates with SBF and ChIPs to the CLN2 promoter.

Figure 6

(A) Cln3 co-immunoprecipitates with Swi6. Strains (HWL72, HWL112, HWL130) with various combinations of CLN3 or CLN3-FLAG (pCM273), SWI6 or SWI6-Myc, or SWI4 or swi4, were grown and extracts made. In the left half of (A, these extracts were tested for the presence of the FLAG-tagged Cln3 and the Myc-tagged Swi6 by Western blotting with Anti-FLAG or Anti-Myc antibody). In the right half, proteins were immunoprecipitated with Anti-Myc antibody (directed against Swi6-Myc), and then these immunoprecipitates were tested by Western blotting for the presence of Swi6-Myc and Cln3-FLAG. (B) As 6A, except that the immunoprecipitation is done with the anti-FLAG antibody, followed by Western analysis with the anti-Myc antibody. (C) A ChIP experiment as in Figure 4B, for association of Cln3-TAP (HWL49, HWL63) with the CLN2 promoter.