Table 2. Plasmids and strains.
Plasmid or Strain | Name | Genotype | Source |
Plasmids | pBA70s | CYC1::lacZ 2 micron URA3 | M. Tyers/B. Andrews |
pMT3579 | SCB-lacZ 2 micron URA3 (“pSCB”) | M. Tyers/B. Andrews | |
pCM273 | TETp-CLN3-6FLAG CEN URA3 | M. Aldea | |
Strains | HWL49 background | ||
HWL49 | MATα leu2 ura3 trp1 his3 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 | H. Wang | |
HWL51 | GAL-CLN3::URA3 bck2::TRP1 cdc34-2 SIN3-TAP | ||
HWL59 | GAL-CLN3::URA3 bck2::TRP1 cdc34-2 RPO21-TAP | ||
HWL61 | GAL-CLN3::URA3 bck2::TRP1 cdc34-2 SWI4-TAP | ||
HWL62 | GAL-CLN3::URA3 bck2::TRP1 cdc34-2 RPD3-TAP | ||
HWL63 | GAL-CLN3-TAP::KanMX bck2::TRP1 cdc34-2 | ||
HWL77 | GAL-CLN3::URA3 bck2::TRP1 cdc34-2 WHI5-TAP | ||
HWL128 | GAL-CLN3::URA3 bck2::TRP1 cdc34-2 STB1-TAP | ||
BF305-15d background | |||
BF305-15d | MATa leu2 his3 ura3 trp1 ade1 arg5,6 met14 | B. Futcher | |
HWL145 | cln3::LEU2 | ||
HWL146 | cln3::LEU2 whi5::KanMX stb1::NAT1 | ||
HWL148 | CLN3::URA3::CLN3 | ||
S288c background | |||
S288c | MATα mal gal2 flo8 | R. Mortimer | |
HWL99 | SIN3-TAP::KanMX whi5::NAT1 | ||
HWL110 | SIN3-TAP::KanMX | ||
HWL117 | WHI5-TAP sin3::NAT1 | ||
HWL119 | SIN3-TAP stb1::NAT1 | ||
W303 background | |||
W303-1a | MATa ade2 trp1 leu2 his3 ura3 can1-100 {psi+} | R. Rothstein | |
HWL72 | tTA::LEU2 | ||
HWL112 | SWI6-13MYC::KanMX4 tTA::LEU2 | ||
HWL130 | SWI6-13MYC::KanMX4 swi4::NAT1 tTA::LEU2 | ||
N497 | cln3::ura3 bck1::TRP1 rme1::hisG | N. Edgington | |
N499 | cln3::ura3 bck1::TRP1 rme1::hisG whi5::KanMX | ||
N451 | cln3::ura3 bck1::TRP1 rme1::hisG stb1::KanMX | ||
N452 | cln3::ura3 bck1::TRP1 rme1::hisG sin3::KanMX | ||
N453 | cln3::ura3 bck1::TRP1 rme1::hisG rpd3::KanMX | ||
N454 | cln3::ura3 bck1::TRP1 rme1::hisG hos3::LEU2 | ||
BY4701/4702 background | |||
OBS1 | MATa his3 leu2 ura3 met15 WHI5-TAP | Open Biosystems | |
OBS5 | MATa his3 leu2 ura3 met15 STB1-TAP | Open Biosystems | |
LC504 | URA3-GAL-CLN3 bck2::KanMX stb1::KanMX whi5::KanMX | L. Carey | |
LC517 | URA3-GAL-CLN3 bck2::KanMX | ||
LC518 | URA3-GAL-CLN3 bck2::KanMX stb1::LEU2 | ||
LC520 | URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 | ||
LC521 | URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 rpd3::KanMX | ||
LC523 | URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 hda2::KanMX | ||
LC524 | URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 sin3::KanMX |
Strains were created during this study unless otherwise indicated. LC strains are from crosses between BY4741 and BY4742 strains. They are his3 leu2 ura3 with MAT, met15 and lys2 segregating.