Skip to main content
. 2009 Sep 8;7(9):e1000189. doi: 10.1371/journal.pbio.1000189

Table 2. Plasmids and strains.

Plasmid or Strain Name Genotype Source
Plasmids pBA70s CYC1::lacZ 2 micron URA3 M. Tyers/B. Andrews
pMT3579 SCB-lacZ 2 micron URA3 (“pSCB”) M. Tyers/B. Andrews
pCM273 TETp-CLN3-6FLAG CEN URA3 M. Aldea
Strains HWL49 background
HWL49 MATα leu2 ura3 trp1 his3 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 H. Wang
HWL51 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 SIN3-TAP
HWL59 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 RPO21-TAP
HWL61 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 SWI4-TAP
HWL62 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 RPD3-TAP
HWL63 GAL-CLN3-TAP::KanMX bck2::TRP1 cdc34-2
HWL77 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 WHI5-TAP
HWL128 GAL-CLN3::URA3 bck2::TRP1 cdc34-2 STB1-TAP
BF305-15d background
BF305-15d MATa leu2 his3 ura3 trp1 ade1 arg5,6 met14 B. Futcher
HWL145 cln3::LEU2
HWL146 cln3::LEU2 whi5::KanMX stb1::NAT1
HWL148 CLN3::URA3::CLN3
S288c background
S288c MATα mal gal2 flo8 R. Mortimer
HWL99 SIN3-TAP::KanMX whi5::NAT1
HWL110 SIN3-TAP::KanMX
HWL117 WHI5-TAP sin3::NAT1
HWL119 SIN3-TAP stb1::NAT1
W303 background
W303-1a MATa ade2 trp1 leu2 his3 ura3 can1-100 {psi+} R. Rothstein
HWL72 tTA::LEU2
HWL112 SWI6-13MYC::KanMX4 tTA::LEU2
HWL130 SWI6-13MYC::KanMX4 swi4::NAT1 tTA::LEU2
N497 cln3::ura3 bck1::TRP1 rme1::hisG N. Edgington
N499 cln3::ura3 bck1::TRP1 rme1::hisG whi5::KanMX
N451 cln3::ura3 bck1::TRP1 rme1::hisG stb1::KanMX
N452 cln3::ura3 bck1::TRP1 rme1::hisG sin3::KanMX
N453 cln3::ura3 bck1::TRP1 rme1::hisG rpd3::KanMX
N454 cln3::ura3 bck1::TRP1 rme1::hisG hos3::LEU2
BY4701/4702 background
OBS1 MATa his3 leu2 ura3 met15 WHI5-TAP Open Biosystems
OBS5 MATa his3 leu2 ura3 met15 STB1-TAP Open Biosystems
LC504 URA3-GAL-CLN3 bck2::KanMX stb1::KanMX whi5::KanMX L. Carey
LC517 URA3-GAL-CLN3 bck2::KanMX
LC518 URA3-GAL-CLN3 bck2::KanMX stb1::LEU2
LC520 URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2
LC521 URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 rpd3::KanMX
LC523 URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 hda2::KanMX
LC524 URA3-GAL-CLN3 bck2::KanMX::HIS3 whi5::LEU2 sin3::KanMX

Strains were created during this study unless otherwise indicated. LC strains are from crosses between BY4741 and BY4742 strains. They are his3 leu2 ura3 with MAT, met15 and lys2 segregating.