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. 2009 Aug 18;2(3):121–127. doi: 10.1593/tlo.09103

Figure 5.

Figure 5

Vascular damage and tumor necrosis after VDA treatment. Panel of digitized images showing tumor (A–D) and normal (E–H) tissue sections obtained from control and DMXAA-treated animals. Control tumors showed a poorly differentiated SCC phenotype (A) and appeared well vascularized with distinctly visible CD31+ endothelial cells (C). In sharp contrast, H&E-stained sections of treated tumors showed widespread areas of necrosis (B). CD31-immunostained sections of tumors obtained from treated animals showed loss of vessel integrity and extensive vascular damage evidenced by minimal or complete absence of CD31 staining (D). H&E-stained sections of salivary glands obtained from both control (E) and treated (F) animals showed normal glandular histology with no tissue necrosis. Corresponding CD31 immunostaining showed no evidence of vascular damage with intact CD31 staining in treated animals (H) similar to controls (G). Digitized images were captured at an apparent magnification of x20 using the ImageScope software (Version 9.1; Aperio Technologies).