Table 1.
Thermodynamic Profiles for the Folding of the Dodecamersa
| [NaCl] (mM) | TM (°C) | ΔHcal (kcal/mol) | TΔScal (kcal/mol) | ΔG°20 (kcal/mol) | ΔnNa+ (mol/mol) |
|---|---|---|---|---|---|
| DDD | |||||
| 10 | 33.3 | -116 | -109 | -6.9 | -2.3 ± 0.2 |
| 100 | 57.7 | -110 | -94.1 | -15 | -1.8 ± 0.1 |
| DDDZ10 | |||||
| 10 | 35.7 | -106 | -99.9 | -6.1 | -1.7 ± 0.1 |
| 100 | 50.0 | -87.7 | -77.0 | -11 | -1.3 ± 0.1 |
| DDD2+ | |||||
| 10 | 29.8 | -68.0 | -64.7 | -3.3 | -1.5 ± 0.1 |
| 100 | 45.3 | -60.0 | -52.1 | -7.9 | -1.2 ± 0.1 |
| DDD2+Z10 | |||||
| 10 | 31.9 | -74.3 | -70.6 | -3.7 | -1.2 ± 0.1 |
| 100 | 42.2 | -70.2 | -61.2 | -9.0 | -1.1 ± 0.1 |
a
All parameters were measured from UV (TM) and DSC melting curves in 10 mM sodium phosphate buffer (pH 7) and at the indicated NaCl concentrations. All TM's correspond to a strand concentration of 10 mM. The experimental uncertainties were as follows: ±0.5 °C for TM, ±5% for ΔHcal, ±7% for ΔG°20, and ±5% for TΔScal.