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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2009 Sep;15(9):1026–1037. doi: 10.1016/j.bbmt.2009.06.002

Fig. 2.

Fig. 2

Culture of CD34+ HPCs in the presence of IL-15 and Notch ligand drives NK cell differentiation, without T or B cell maturation. Flow cytometric analysis of 4 week-old cultures containing the Notch ligand Delta4 and IL-15 demonstrate abundance of CD7+CD56+ NK cells and no T cell precursors (A), mature T cells (CD3+; B), or B cells (CD19+; C), while culture without IL-15 in the presence of Delta4 demonstrates predicted T cell precursors (CD1a+CD7+; A). Culture without Notch ligand (RET-10) in the presence of IL-15 demonstrates few CD7+ NK cell precursors and CD56+ NK cells (A, B), with mostly CD33+ myeloid cells and no development of T or B cells (A–C). All cultures contained IL-7 and FltL. Data from cultures containing the Notch ligands Jagged2 or Delta1 showed similar results to the Delta4 culture. Data shown is representative of at least 4 experiments.