Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Struct Biol. 2009 Mar 24;166(3):303–315. doi: 10.1016/j.jsb.2009.03.009

Figure 4. Cyto1-EpsF inhibits secretion of protease via the T2SS in V. cholerae.

Figure 4

Wild type V. cholerae containing either pMMB67 as negative control or pMMB-cyto1-EpsF was grown in LB in the absence or presence of increasing concentrations of IPTG to induce the expression of cyto1-EpsF. Culture supernatants and cells were separated by centrifugation. (A) Cells were disrupted and subjected to SDS-PAGE and immunoblotting with anti-EpsF antibodies to determine the relative level of cyto1-EpsF expression at different IPTG concentrations. The position of molecular mass markers is shown on the left and native EpsF and cyto1-EpsF are indicated on the right. (B) Culture supernatants were tested for the presence of extracellular protease. The rate of hydrolysis was obtained from three independent experiments and the results are presented as percent extracellular activity ± standard error (SEM). The extracellular activity in the supernatant from the negative control was set to 100%. The epsF mutant, which is unable to secrete the protease, is shown as positive control.