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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Struct Biol. 2009 Mar 24;166(3):303–315. doi: 10.1016/j.jsb.2009.03.009

Figure 5. Interaction of cyto1-EpsF and EpsL.

Figure 5

EpsL was co-purified with cyto1-EpsFhis6 from V. cholerae cell extracts by metal-affinity chromatography and subjected to SDS-PAGE and immunoblotting with anti-EpsL antibodies. Lanes 1-4 show the level of EpsL detected in cell extracts prior to purification, and lanes 5-8 represent EpsL proteins that bound to hexahistidine-tagged cyto1-EpsF. No EpsL was purified when cyto1-EpsF was not expressed (lanes 5 and 6). Positions of molecular weight markers are shown.