Dose and time response of (a) Syt-7 and (b) LAMP1 mRNA expression in naïve wild-type Mφ treated with PGE2. (c) Syt-7 and LAMP1 expression in wild-type Mφ infected or not with H37Rv after addition of PGE2 (1 μM). (d) Syt-7 and LAMP1 mRNA expression in Alox5−/−, Ptges−/− and wild-type (WT) Mφ at indicated times after infection with H37Rv. (e) Wild-type mice were infected or not by the aerosol route with a low dose (~ 100 CFU) of H37Rv or H37Ra. After 7 days of infection, RNA was extracted from the whole lung and the expression of Syt-7 and LAMP1 mRNA was measured by real-time PCR. The expression of Syt-7 or LAMP1 was normalized to β-Actin. Results are from one representative of 2 (a, b, c, and f) and 3 (d, e) independent experiments. n=3 mice per group; (error bars, s.e.m.) *, p < 0.05.