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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Immunol. 2009 Jun 28;10(8):899–906. doi: 10.1038/ni.1758

Figure 7. Syt-7 is essential for induction of plasma membrane repair, prevention of necrosis, and control of bacterial growth in murine Mφ.

Figure 7

(a,b) Translocation of LAMP1 to the cell surface of Alox5−/−, Ptges−/−, and wild-type (WT) Mφ left uninfected or 24 h after infection with H37Rv (Mtb; MOI 5:1) as analyzed by flow cytometry (a) or confocal microscopy (b). In (b) Mφ were infected with GFP-labeled H37Rv (MOI 10:1), and nonpermeabilized cells were stained with a monoclonal antibody against the lumenal domain of LAMP1. Scale bar 5 μm. (c) Alox5−/− Mφ were left untreated or were transfected with scrambled (Scr) siRNA or siRNA specific for Syt-7 for 24 h followed by H37Rv infection (MOI 5:1). Apoptosis and necrosis of the treated and infected Mφ compared to uninfected controls was measured 3 days after infection. (d) H37Rv growth was measured in Alox5−/− and WT Mφ left untreated or transfected with Syt-7-specific or scrambled (Scr) siRNA at the indicated times after infection. Results are representative of 2 independent experiments. (error bars, s.e.m.) *, p < 0.05.