Fig. 2.
(A) ETS induces NF-κB activation in mice brain. ETS increases NF-κB DNA binding in mice brain. Twenty micrograms nuclear proteins were analyzed in 7.4% native PAGE to detect NF-κB by electrophoretic mobility shift assay. The data shown is a representative of five independent experiments performed on extracts obtained from control and ETS exposed mice brain regions. (B) Supershift and specificity of NF-κB activation. Brain tissue extracts prepared from brain region of control and ETS exposed mice were incubated for 15 min with different antibodies, cold and mutant NF-κB oligonucleotides, and then assayed for NF-κB, as described in Section 2. The data shown is a representative of five independent experiments performed on extracts obtained from control and ETS exposed mice brain regions.